LONG-TERM TRACING OF VITAL NEURONS WITH HOECHST-33342 IN TRANSPLANTATION STUDIES

被引：12

作者：

SPRICK, U

机构：

[1] C. and O. Vogt-Institute of Brain Research, Heinrich-Heine-Universität Düsseldorf, D-4000 Düsseldorf

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1991年 / 36卷 / 2-3期

关键词：

BISBENZIMIDE (HOECHST-33342); VITAL TRACER; LONG-TERM GRAFTING; NEURONAL TISSUE;

D O I：

10.1016/0165-0270(91)90049-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hippocampal nerve cells of rats taken from embryonic donors were stained with the fluorescent dye bisbenzimide (Hoechst 33342) and grafted to the brains of adult rats. The numbers of labelled neurons inside the transplants were determined after 6 different intervals, ranging from 2 days to 6 months. Numerous labelled neurons were found inside the grafts up to 6 months after grafting. The results demonstrate that bisbenzimide is a suitable vital cell tracer for long-term grafting experiments.

引用

页码：229 / 238

页数：10