GENETIC MICRO-SURGERY BY LASER - ESTABLISHMENT OF A CLONAL POPULATION OF RAT KANGAROO CELLS (PTK2) WITH A DIRECTED DEFICIENCY IN A CHROMOSOMAL NUCLEOLAR ORGANIZER

被引：25
作者：
BERNS, MW
CHONG, LK
HAMMERWILSON, M
MILLER, K
SIEMENS, A
机构：
[1] Developmental and Cell Biology, University of California, Irvine, Irvine, 92717, California
来源：
CHROMOSOMA | 1979年 / 73卷 / 01期
关键词：
D O I：
10.1007/BF00294839
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells. © 1979 Springer-Verlag.
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页码：1 / 8
页数：8
相关论文
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