IDENTIFICATION OF A SPECIFIC PATTERN OF VASCULAR ENDOTHELIAL GROWTH-FACTOR MESSENGER-RNA EXPRESSION IN HUMAN PLACENTA AND CULTURED PLACENTAL FIBROBLASTS

被引：59

作者：

ANTHONY, FW ^{[1
]}

WHEELER, T ^{[1
]}

ELCOCK, CL ^{[1
]}

PICKETT, M ^{[1
]}

THOMAS, EJ ^{[1
]}

机构：

[1] SOUTHAMPTON GEN HOSP,DEPT MOLEC MICROBIOL,SOUTHAMPTON SO9 4XY,HANTS,ENGLAND

来源：

PLACENTA | 1994年 / 15卷 / 05期

关键词：

D O I：

10.1016/S0143-4004(05)80424-2

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reverse transcription and the polymerase chain reaction (PCR) were used to detect vascular endothelial growth factor (VEGF) mRNA in human placental tissue and cultured placental fibroblasts obtained during the first trimester of pregnancy. The primers for VEGF corresponded to areas in exon 4 and exon 8 of the VEGF gene. After one round of PCR three products, equivalent to VEGF121, VEGF165 and VEGF189, were detected within placental tissue and cultured placental fibroblasts. A further round of PCR revealed the presence of two more products equivalent to VEGF206 and VEGF145. Thus, in addition to the production of readily secreted form of VEGF (VEGF121 and VEGF165), the placenta produces several transcripts expected to increase the growth factor pool of the extracellular matrix. © 1994, W. B. Saunders Company Ltd.. All rights reserved.

引用

页码：557 / 561

页数：5

共 10 条

[1] IDENTIFICATION AND LOCALIZATION OF ALTERNATELY SPLICED MESSENGER-RNAS FOR VASCULAR ENDOTHELIAL GROWTH-FACTOR IN HUMAN UTERUS AND ESTROGEN REGULATION IN ENDOMETRIAL CARCINOMA CELL-LINES [J].

CHARNOCKJONES, DS ;

SHARKEY, AM ;

RAJPUTWILLIAMS, J ;

BURCH, D ;

SCHOFIELD, JP ;

FOUNTAIN, SA ;

BOOCOCK, CA ;

SMITH, SK .

BIOLOGY OF REPRODUCTION, 1993, 48 (05) :1120-1128

[2] PITUITARY FOLLICULAR CELLS SECRETE A NOVEL HEPARIN-BINDING GROWTH-FACTOR SPECIFIC FOR VASCULAR ENDOTHELIAL-CELLS [J].

FERRARA, N ;

HENZEL, WJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (02) :851-858

[3] THE VASCULAR ENDOTHELIAL GROWTH-FACTOR FAMILY OF POLYPEPTIDES [J].

FERRARA, N ;

HOUCK, KA ;

JAKEMAN, LB ;

WINER, J ;

LEUNG, DW .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1991, 47 (03) :211-218

[4] ISOLATION AND CHARACTERIZATION OF A VASCULAR ENDOTHELIAL-CELL MITOGEN PRODUCED BY PITUITARY-DERIVED FOLLICULO STELLATE CELLS [J].

GOSPODAROWICZ, D ;

ABRAHAM, JA ;

SCHILLING, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (19) :7311-7315

[5] THE VASCULAR ENDOTHELIAL GROWTH-FACTOR FAMILY - IDENTIFICATION OF A 4TH MOLECULAR-SPECIES AND CHARACTERIZATION OF ALTERNATIVE SPLICING OF RNA [J].

HOUCK, KA ;

FERRARA, N ;

WINER, J ;

CACHIANES, G ;

LI, B ;

LEUNG, DW .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (12) :1806-1814

[6]

HOUCK KA, 1992, J BIOL CHEM, V267, P26031

[7]

RODESCH F, 1992, OBSTET GYNECOL, V80, P283

[8] VASCULAR ENDOTHELIAL GROWTH-FACTOR INDUCED BY HYPOXIA MAY MEDIATE HYPOXIA-INITIATED ANGIOGENESIS [J].

SHWEIKI, D ;

ITIN, A ;

SOFFER, D ;

KESHET, E .

NATURE, 1992, 359 (6398) :843-845

[9]

TISCHER E, 1991, J BIOL CHEM, V266, P11947

[10] REVERSE TRANSCRIPTION WITH NESTED POLYMERASE CHAIN-REACTION SHOWS EXPRESSION OF BASIC FIBROBLAST GROWTH-FACTOR TRANSCRIPTS IN HUMAN GRANULOSA AND CUMULUS CELLS FROM INVITRO FERTILIZATION PATIENTS [J].

WATSON, R ;

ANTHONY, F ;

PICKETT, M ;

LAMBDEN, P ;

MASSON, GM ;

THOMAS, EJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 187 (03) :1227-1231

← 1 →