Implementation of a semi-automated processing system for DNA profiling of forensic casework samples

被引：4

作者：

Vollack, Kai ^{[1
]}

Haak, Barbara ^{[1
]}

Schwenzer, Ralph ^{[1
]}

Pflug, Werner ^{[1
]}

机构：

[1] Landeskriminalamt Baden Wurttemberg Off Criminal, Taubenheimstr 85, D-70372 Stuttgart, Germany

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2008年 / 1卷 / 01期

关键词：

DNA profiling; Semi-automated processing; Laboratory data network;

D O I：

10.1016/j.fsigss.2007.11.006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The concept for a semi-automated processing system for DNA analysis of crime scene samples was developed at the Landeskriminalamt Baden-Wurttemberg (LKA BW) and comprises the extraction of genomic DNA from human cells by ChargeSwitch((R)) magnetic bead technology (CST), quantification of purified DNA by real-time PCR, amplification of short tandem repeats (STRs) by PCR and DNA fragment length analysis of STRs by capillary electrophoresis. Three liquid handling workstations from Tecan, a real-time PCR device and a 16-channel capillary electrophoresis (CE) system, both from Applied Biosystems (AB), are linked via laboratory data network. Transmission and management of sample and analysis data is enabled by a Laboratory Information and Management System (LIMS). Suitability for a wide range of stain types, early exclusion of DNA-free samples, barcode sample identification and prevention of cross-contaminations guarantee efficiency and high quality standards. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：83 / 85

页数：3

共 2 条

[1] Evaluation of a semi-automated, magnetic bead-based DNA extraction method for genetic fingerprinting of forensic casework samples [J].

Haak, Barbara ;

Porsche, Andrea ;

Vollack, Kai ;

Zimmermann, Peter ;

Pflug, Werner .

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2008, 1 (01) :35-36

[2] Implementation of a robotized real-time PCR setup for the use of the Quantifiler (TM) Human DNA Quantification Kit [J].

Schwenzer, Ralph ;

Schoeneberg, Annette ;

Pflug, Werner .

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2008, 1 (01) :68-70

← 1 →