TRANSFORMATION OF 4 PATHOGENIC PHYTOPHTHORA SPP BY MICROPROJECTILE BOMBARDMENT ON INTACT MYCELIA

被引：21
作者：
BAILEY, AM
MENA, GL
HERRERAESTRELLA, L
机构：
[1] Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados (CINVESTAV), Unidad Irapuato, Irapuato, 36500, Guanajuato
来源：
CURRENT GENETICS | 1993年 / 23卷 / 01期
关键词：
PHYTOPHTHORA; TRANSFORMATION; BIOLISTIC; BETA-GLUCURONIDASE; PLANT PROMOTER;
D O I：
10.1007/BF00336748
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the beta-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric beta-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.
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收藏
页码：42 / 46
页数：5
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