TRANSFORMATION OF 4 PATHOGENIC PHYTOPHTHORA SPP BY MICROPROJECTILE BOMBARDMENT ON INTACT MYCELIA

被引：22

作者：

BAILEY, AM

MENA, GL

HERRERAESTRELLA, L

机构：

[1] Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados (CINVESTAV), Unidad Irapuato, Irapuato, 36500, Guanajuato

来源：

CURRENT GENETICS | 1993年 / 23卷 / 01期

关键词：

PHYTOPHTHORA; TRANSFORMATION; BIOLISTIC; BETA-GLUCURONIDASE; PLANT PROMOTER;

D O I：

10.1007/BF00336748

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Phytophthora capsici, P. citricola, P. cinnamomi and P. citrophthora were transformed without the removal of cell walls by particle acceleration with plasmids containing the beta-glucuronidase gene and hygromycin B resistance. Transformants were detected by histochemical and fluorometric beta-glucuronidase assays and confirmed by Southern-blot hybridization. It was found that the promoter of a plant virus is functional in Phytophthora. In addition, a method was designed to visually identify homogeneous transformed colonies, derived from zoospores of transformed multinucleated Phytophthora mycelia, based on blue color development on plates containing X-Gluc.

引用

页码：42 / 46

页数：5

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