PRESENCE OF ESTROGEN-BINDING SITES ON MACROPHAGE-LIKE SYNOVIOCYTES AND CD8+, CD29+, CD45RO+ T-LYMPHOCYTES IN NORMAL AND RHEUMATOID SYNOVIUM

被引:94
作者
CUTOLO, M
ACCARDO, S
VILLAGGIO, B
CLERICO, P
BAGNASCO, M
COVIELLO, DA
CARRUBA, G
LOCASTO, M
CASTAGNETTA, L
机构
[1] NATL INST CANC RES GENOVA,HORMONE BIOCHEM LABS,PALERMO,ITALY
[2] UNIV GENOA,DEPT INTERNAL MED,DIV NEPHROL,I-16132 GENOA,ITALY
[3] UNIV GENOA,DEPT INTERNAL MED,DIV CLIN IMMUNOL,I-16132 GENOA,ITALY
[4] UNIV GENOA,INST BIOL & GENET,I-16132 GENOA,ITALY
[5] UNIV TURIN,DEPT ORTHOPED SURG,I-10124 TURIN,ITALY
[6] UNIV PALERMO,HORMONE BIOCHEM LABS,I-90134 PALERMO,ITALY
[7] NATL INST CANC RES GENOVA,MOLEC ENDOCRINOL SECT,PALERMO,ITALY
来源
ARTHRITIS AND RHEUMATISM | 1993年 / 36卷 / 08期
关键词
D O I
10.1002/art.1780360809
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. To study the presence of estrogen-binding sites (EBS) in the synovial tissues of male and female patients with rheumatoid arthritis (RA) and in age- and sex-matched healthy controls. Methods. Both type 1 (high affinity, low binding capacity) and type 2 (reduced affinity, higher binding capacity) EBS were investigated in both soluble and nuclear fractions of homogenized synovial tissue samples by a dextran-coated charcoal method. To determine what type of synovial cell was positive for EBS, cryosections of synovial tissues were immunostained with a specific monoclonal anti-estrogen receptor antibody (anti-ER MAb) using both immunofluorescence and immunoperoxidase techniques. Double immunostaining with the anti-ER MAb and with specific MAb to detect different macrophage antigens (Ber-MAC3, MAC387, CD68) and CD8+ T cell subsets (CD29+, CD45RO+ and CD29-, CD45RO-) was performed. Results. Higher affinity EBS were found mostly in nuclear cell fractions of either RA or control synovial tissues (28 of the 33). These EBS were present to a lesser extent in soluble cell fractions (11 of the 33). Immunostaining showed the estrogen receptor-positive cells to be the macrophage-like synoviocytes and the CD8+, CD29+ T cells both in RA and in control synovial tissues. Higher nuclear content of EBS was consistent with more intense nuclear staining of synoviocytes and T cells. Conclusion. It is conceivable that the immunomodulatory activity exerted by estrogens is at least partly mediated through their interaction with EBS that are present on macrophage-like synoviocytes, functioning as antigen-processing and antigen-presenting cells, and on antigen-experienced (memory) CD8+ T lymphocytes (CD29+, CD45RO+).
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页码:1087 / 1097
页数:11
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