DOLICHOL AND POLYPRENOL KINASE-ACTIVITIES IN MICROSOMES FROM ETIOLATED RYE SEEDLINGS

被引：6

作者：

RYMERSON, RT ^{[1
]}

CARROLL, KK ^{[1
]}

RIP, JW ^{[1
]}

机构：

[1] CHILDRENS PSYCHIAT RES INST,LONDON N6H 3W7,ONTARIO,CANADA

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1992年 / 70卷 / 06期

关键词：

DOLICHOL; POLYPRENOL; DOLICHYL PHOSPHATE; POLYPRENYL PHOSPHATE; KINASES;

D O I：

10.1139/o92-069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dolichol kinase activity in microsomes from etiolated rye seedlings had a pH optimum at 8 with a shoulder at pH 6.5. Triton X-100 (0.4%) was required for optimum activity. Exogenous divalent cations did not enhance activity, although Mg+2 was added routinely. Rye microsomes were found to contain dolichol and polyprenol in a ratio of 3 to 2. Rye, soybean embryo, and rat liver microsomes catalyzed the synthesis of 78, 52, and 516 nmol [C-14]dolichyl phosphate/(mg microsomal protein . h) compared with 21, 22, and 49 nmol [H-3]polyprenyl phosphate/(mg microsomal protein.h), respectively. It is clear that microsomes from plant systems can catalyze the phosphorylation of polyprenol better than rat liver when compared with their abilities to catalyze the phosphorylation of dolichol. It is not known whether one or more kinases is responsible for catalyzing the phosphorylation of these two closely related groups of compounds.

引用

页码：455 / 459

页数：5