SUBUNITS OF RNA-POLYMERASE IN FUNCTION AND STRUCTURE .11. IDENTIFICATION OF A SUBUNIT ASSEMBLY DOMAIN IN THE ALPHA-SUBUNIT OF ESCHERICHIA-COLI RNA-POLYMERASE

被引：80
作者：
IGARASHI, K
FUJITA, N
ISHIHAMA, A
机构：
[1] Department of Molecular Genetics National Institute of Genetics Mishima, Shizuoka
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 218卷 / 01期
关键词：
D O I：
10.1016/0022-2836(91)90865-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The α subunit of Escherichia coli DNA-dependent RNA polymerase is encoded by the rpoA gene and plays a major role in enzyme assembly. A set of C-terminal deletion mutations of the rpoA gene was constructed. The results of mixed reconstitution experiments in vitro, using the truncated α polypeptides encoded by the rpoA deletion mutants, suggest that the amino-terminal two-thirds of α subunit is sufficient for the formation of pseudo-core complexes containing both β and β′ subunits. © 1991.
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页码：1 / 6
页数：6
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