IDENTIFICATION OF THE PHOTOSYSTEM-I ANTENNA POLYPEPTIDES IN BARLEY - ISOLATION OF 3 PIGMENT-BINDING ANTENNA COMPLEXES

被引:77
作者
KNOETZEL, J [1 ]
SVENDSEN, I [1 ]
SIMPSON, DJ [1 ]
机构
[1] CARLSBERG LAB,DEPT CHEM,DK-2500 COPENHAGEN,DENMARK
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 206卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1992.tb16918.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The light-harvesting antenna of barley photosystem I (LHCI) was isolated from native photosystem I (PSI) complexes and fractionated into three pigment-protein subcomplexes using two consecutive rounds of green gel electrophoresis. Each complex showed a characteristic polypeptide composition and low-temperature fluorescence emission spectrum; they were designated as LHCI-730, LHCI-680A and LHCI-680B. Their four apoproteins of 21, 22, 23 and 25 kDa were purified and NH2-terminal sequences were determined; in the case of the NH2-terminally blocked 25-kDa protein, an internal sequence was obtained after cleavage with endoproteinase Lys-C. This made possible an assignment of the four proteins to the four types (I - IV) of genes coding for chlorophyll alb proteins of PSI (cab or lha genes). The LHCI-730 complex was isolated as a heterodimer composed of the 21-kDa (LHCI type IV) and the 22-kDa (LHCI type I) polypeptides. Each LHCI-680 complex had a single apoprotein. LHCI-680A consisted of the 25-kDa (LHCI type III) and LHCI-680B of the 23-kDa (LHCI type II) polypeptides. LHCI-680B was associated with the non-pigmented PSI-E subunit, indicating that this protein may function in the binding of this antenna to the reaction centre.
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页码:209 / 215
页数:7
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