LYSOLECITHIN ANALOGS AS ADJUVANTS IN DELAYED-TYPE HYPERSENSITIVITY IN MICE .1. CHARACTERIZATION OF THE ADJUVANT EFFECT

The adjuvant activity of 5 different lysolecithin analogs (LLA) has been studied in delayed‐type hypersensitivity (DTH) using fowl gamma globulin, bovine serum albumin and the terpolymer L‐glutamic acid 60‐L‐alanine30‐L‐tyrosine10(GAT), as antigens. Increased DTH responses, by factors of 1.8–2.0 in CBA and BALB/c mice, showed that LLA are immunopotentiators if given intraperitoneally (i.p.) or subcutaneously (s.c.) together with the antigen. The concentration range, within which LLA are active, is limited to 10–20 (μg/mouse s.c. and 100–300 μg/mouse i.p., Adjuvanticity was tested as a function of the LLA structure. The most pronounced immunopotentiation was obtained with racemic 1‐octadecyl‐2‐methylglycero‐3‐phosphorylcholine (ET18‐O‐CH3). The LLA became less active with decreasing numbers of C atoms in the alkyl chain. Copyright © 1979 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim