PURIFICATION AND PROPERTIES OF A 155-KDA BONE MARROW-DERIVED GLYCOPROTEIN ENHANCING THE ACTIVITY OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR-(GM-CSF)

被引：0

作者：

KATLINSKY, AV

PETROV, RV

MIKHAILOVA, AA

LAERUM, OD

机构：

[1] UNIV BERGEN,HAUKELAND HOSP,GADE INST,DEPT PATHOL,N-5021 BERGEN,NORWAY

[2] MM SHEMYAKIN BIOORGAN CHEM INST,DEPT IMMUNOL,MOSCOW 117871,RUSSIA

来源：

FEBS LETTERS | 1993年 / 320卷 / 01期

关键词：

GM-CSF; BONE MARROW; COLONY FORMATION ENHANCEMENT;

D O I：

10.1016/0014-5793(93)81659-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein has been purified from pig bone marrow which enhances the activity of exogenously added granulocyte-macrophage colony stimulating factor (GM-CSF) on mouse myelopoietic colony formation in vitro. The substance alone did not exhibit any colony stimulating activity. The active fraction was isolated from the medium of pig bone marrow suspension cultures during 20-24 h. Purification to homogeneity was then performed by subsequent HPLC and SDS-PAGE. The GM-CSF enhancing protein was identified as a 155 kDa glycoprotein.

引用

页码：67 / 70

页数：4

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