PREDICTING PARTITION-COEFFICIENTS IN POLYETHYLENE-GLYCOL POTASSIUM PHOSPHATE AQUEOUS 2-PHASE SYSTEMS

被引：32
作者：
EITEMAN, MA [1 ]
GAINER, JL [1 ]
机构：
[1] UNIV VIRGINIA, DEPT CHEM ENGN, CTR BIOPROC & PROD DEV, CHARLOTTESVILLE, VA 22903 USA
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 586卷 / 02期
关键词：
D O I：
10.1016/0021-9673(91)85142-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Partition coefficients in polyethylene glycol-potassium phosphate aqueous two-phase systems are predicted using a previously developed mathematical model. The model is based on a simplification of equations which arise from an osmotic pressure virial expansion and relates the partition coefficient to the concentration difference between phases of one of the phase-forming components and to the solute hydrophobicity. The predicted partition coefficients are compared to experimental values for several different solutes in this phase system over the range of pH of 5.5 to 9.2. The predictions are generally good for uncharged solutes, but show disagreement with experimental values for charged solutes.
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页码：341 / 346
页数：6
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