CONTRIBUTION OF THE CRYOPROTECTANT TO TOTAL INJURY IN RABBIT HEARTS FROZEN WITH ETHYLENE-GLYCOL

被引：20

作者：

ARMITAGE, WJ

PEGG, DE

机构：

[1] MRC Medical Cryobiology Group, University Department of Surgery, Cambridge, CB2 2AH England, Douglas House, Trumpington Rd

来源：

CRYOBIOLOGY | 1979年 / 16卷 / 02期

关键词：

D O I：

10.1016/0011-2240(79)90025-7

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Following the failure of hearts to recover function after freezing at -20 ° in the presence of 3 m ethylene glycol, a variety of experimental treatments was devised to determine the relative harmfulness of ice, high concentrations of electrolytes and high ethylene glycol concentration. Neither cooling to -20 °C without freezing in a Ca2+-free solution containing twice the normal salt concentration and 6 m ethylene glycol (freezing 3 m ethylene glycol at -20 °C doubles the solute concentration in the liquid phase), nor perfusion at -1 °C with this solution were conducive to the recovery of hearts. However, perfusion with Ca2+-free 3 m ethylene glycol solution with twice the normal concentration of salts did allow full recovery of function, whereas perfusion with Ca2+-free 6 m ethylene glycol solution with normal salt concentrations did not. Therefore, the high ethylene glycol concentration encountered during freezing was the main cause of damage. © 1979.

引用

页码：152 / 160

页数：9

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