RETINOIC ACID - ENDOGENOUS COMPOUND OF HUMAN-BLOOD - UNEQUIVOCAL DEMONSTRATION OF ENDOGENOUS RETINOIC ACID IN NORMAL PHYSIOLOGICAL CONDITIONS

被引：127

作者：

DERUYTER, MG ^{[1
]}

LAMBERT, WE ^{[1
]}

DELEENHEER, AP ^{[1
]}

机构：

[1] STATE UNIV GHENT,FAC FARMACEUT,MED BIOCHEM KLIN ANAL LAB,B-9000 GHENT,BELGIUM

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90159-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The occurrence of retinoic acid (vitamin A acid) as a normal constituent of the vitamin A reserve from the body is demonstrated. Improved methodologies based on selected peak monitoring (spm) and high-performance liquid chromatography (hplc) are used for the detection of retinoic acid in EDTA-plasma. After extensive cleanup by double-phase extraction and chromatography on Sephadex LH-20, retinoic acid is determined as its methyl derivative by spm. A different approach using double-phase extraction combined with a preextraction step and hplc is used to confirm the findings of the spm experiments. Both techniques proved the presence of retinoic acid in human plasma at a concentration of 1 to 3 ng/ml. © 1979.

引用

页码：402 / 409

页数：8

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