RAPID SEPARATIONS BY LIQUID-SOLID COLUMN CHROMATOGRAPHY . QUALITATIVE AND QUANTITATIVE ANALYSIS OF HYDROGENATED QUINOLINE MIXTURES

Liquid-solid column chromatography (LSCC) with optimized solvent programming was applied to the qualitative identification of the components of hydrogenated quinoline samples. Quinoline, 1,2,3,4-and 5,6,7,8-tetrahydroquinolines, and o-propylaniline were present in major amounts, along with several minor components of the genera) formula C18, H17-27 N. A rapid LSCC procedure for the routine determination of these sample components was developed. Several hundred such analyses were carried out on a single column over a period of six months. Column performance was maintained at essentially the starting level by attention to the water content of the solvent plus occasional column regeneration to remove strongly held sample impurities. The present study illustrates some of the problems likely to be encountered in routine LSCC applications and points the way to their solution. © 1969, Oxford University Press. All rights reserved.