A tissue culture practical is described which uses vegetable Brassica seedlings germinated in-vitro and cut up into root, hypocotyl, and cotyledon explants. These are then cultured on media containing different levels of phytohormones which induce shoot, root, and callus production in as little as Four to six weeks. The practical is simple and easy to use and does not require the use of a lamina flow cabinet. Further developments from the basic schedule are suggested.
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Periyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, IndiaPeriyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, India
Balamurugan, Venkatachalam
Abdi, Gholamreza
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Persian Gulf Univ, Persian Gulf Res Inst, Dept Biotechnol, Bushehr 75169, IranPeriyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, India
Abdi, Gholamreza
Karthiksaran, Chinnasamy
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Periyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, IndiaPeriyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, India
Karthiksaran, Chinnasamy
Thillaigovindhan, Nedumaran
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Sir Theagaraya Coll, Dept Plant Biol & Plant Biotechnol, Chennai 600021, Tamil Nadu, IndiaPeriyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, India
Thillaigovindhan, Nedumaran
Arulbalachandran, Dhanarajan
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Periyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, IndiaPeriyar Univ, Sch Life Sci, Dept Bot, Div Crop Mol Breeding & Stress Physiol, Salem 636011, Tamil Nadu, India