Study of Antioxidant and Antimicrobial Activity of Leaves and Twigs Extracts and Fraction of Jatropha curcas L. and Its Utilization in Personal Hygiene Products

The development of jarak pagar (Jatropha curcas L) in Indonesia needs to be followed by the optimum utilization of all parts of the plants, including the leaves and twigs. This study aims to determine the antioxidant and antimicrobial activities of the leaves and twigs extracts of Jatropha curcas L and to see the effects of their use in personal hygiene products, as part of efforts to increase the added value of the Jatropha's leaves and twigs. Extract of Jatropha curcas in this study were obtained through a solvent extraction process using ethanol 96% by the method of soxhlet and maceration. Fraction obtained by performing advanced stages in the extract called fractination. Antioxidant activity was analized by using DPPH method at concentration of 4, 6, 8, 10, 12, 16 and 20 mu g/mL. While the analysis of antimicrobial activity was performeed by well diffusion method against Candida albicans, Microsporum gypseum, and Pseudomonas aeruginosa at the concentration of extract/fraction 1, 2, and 3%. The result showed that crude extract of maceration and ethyl acetate fraction of soxhlet had the highest antioxidant activity with IC50 values of 7.019 and 7.857 mu g/mL where this values were not significantly different. In other side, the result of antimicrobial activity indicated the present of antimicrobial inhibitory of maceration crude extract against Microsporum gypseum. It proved by the formation of clear zones of inhibition around the wells with diameter of 12, 14, and 20 mm for concentration 1, 2, and 3%. The ethyl acetate fraction showed the inhibitory against Microsporum gypseum only at concentration of 3% extract with diameter of inhibitory zones 14 mm. Utilization of selected extract fraction applied to one of personal hygiene product, transparent soap, by utilizing its antioxidant activity. Soap with the addition of ethyl acetate fraction of Jatropha curcas at a concentration level of 0.8% produces soap with antioxidant activity of 66.15% and the rate of foam stability about 83.23%.