HEME OXYGENASE IS A HEAT-SHOCK PROTEIN AND PEST PROTEIN IN RAT ASTROGLIAL CELLS

被引：83

作者：

DWYER, BE

NISHIMURA, RN

DEVELLIS, J

YOSHIDA, T

机构：

[1] VET AFFAIRS MED CTR,INVITRO REMYELINAT LAB,SEPULVEDA,CA 91343

[2] UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024

[3] UNIV CALIF LOS ANGELES,SCH MED,DEPT ANAT & PSYCHIAT,LOS ANGELES,CA 90024

[4] UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024

[5] UNIV CALIF LOS ANGELES,SCH MED,MENTAL RETARDAT RES CTR,LOS ANGELES,CA 90024

[6] YAMAGATA UNIV,SCH MED,DEPT MOLEC & PATHOL BIOCHEM,YAMAGATA 990,JAPAN

来源：

GLIA | 1992年 / 5卷 / 04期

关键词：

STRESS PROTEIN; CANCER; BRAIN; OXIDATIVE INJURY;

D O I：

10.1002/glia.440050407

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cultured rat forebrain astrocytes contained significant amounts of immunostainable heme oxygenase-1 (HO-1) isozyme, whereas HO-1 was undetectable in spontaneously transformed rat astroglial cells (ATs). HO-1 was inducible in both cell types by heat shock and by submicromolar amounts of H2O2. Inhibition of RNA synthesis with actinomycin D or protein synthesis with cycloheximide resulted in the rapid loss of immunostainable heme oxygenase in astrocytes. Analysis of the primary structure of heme oxygenase suggests that it is a PEST protein, i.e., targeted for rapid turnover.

引用

页码：300 / 305

页数：6

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