A SIMPLIFIED NAUTA PROCEDURE

被引：34
作者：
EBBESSON, SO
RUBINSON, K
机构：
来源：
PHYSIOLOGY & BEHAVIOR | 1969年 / 4卷 / 02期
关键词：
Brain; Staining method;
D O I：
10.1016/0031-9384(69)90096-1
中图分类号：
B84 [心理学];
学科分类号：
04 ; 0402 ;
摘要：
An improved and simplified procedure for the selective silver impregnation of degenerating axons is described. The modification consists of soaking the sections in 0.01% potassium permanganate overnight after treatment in a 5% uranyl nitrate solution. This pretreatment apparently requires no modification for different brains, as did the original technique. This standardized method has been used successfully in studies on sharks, teleosts, amphibians, reptiles, birds, and mammals. Some advantages of the procedure are that the impregnation of degenerating axoplasm is enhanced, the results are easily reproducible, and technician time is substantially reduced. © 1969.
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页码：281 / &
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