THERMOSTABLE EXTRACELLULAR PROTEASE OF BACILLUS-STEAROTHERMOPHILUS - FACTORS AFFECTING ITS PRODUCTION

被引：23

作者：

RAZAK, NA ^{[1
]}

SAMAD, MYA ^{[1
]}

BASRI, M ^{[1
]}

YUNUS, WMZW ^{[1
]}

AMPON, K ^{[1
]}

SALLEH, AB ^{[1
]}

机构：

[1] UNIV PERTANIAN MALAYSIA,FAC SCI & ENVIRONM STUDIES,ENZYME & MICROBIAL TECHNOL GRP,SERDANG 43400,MALAYSIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1994年 / 10卷 / 03期

关键词：

BACILLUS-STEAROTHERMOPHILUS; PROTEASE;

D O I：

10.1007/BF00414858

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A strain of protease-producing Bacillus stearothermophilus has been isolated. Glycerol was the best carbon source for production whereas yeast extract was the best nitrogen source. The bacterium could grow up to 70-degrees-C but optimum protease production was at 60-degrees-C. Best initial pH for protease production was 5. Alkaline pH inhibited production. The enzyme was stable at 60-degrees-C for 18 h and was inhibited by EDTA, PMSF and HgCl2.

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收藏

页码：260 / 263

页数：4

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