HIGH-PERFORMANCE ELECTROPHORESIS INCLUDING SEPARATION OF NUCLEIC-ACIDS AND THEIR DEGRADATION PRODUCTS - INTERPLAY BETWEEN THEORY AND PRACTICAL EXPERIMENTS

被引:6
作者
HJERTEN, S
机构
[1] Department of Biochemistry, University of Uppsala, Biomedical Center, Uppsala
来源
NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 03期
关键词
D O I
10.1080/07328319008045143
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The analogy between electrophoresis, centrifugation and chromatography is stressed by consideration of a formula which is valid for all three methods. An equation for electrophoretic plate heights forms the basis for a discussion of the experimental conditions for maximum resolution. The difference between true and apparent plate numbers and plate heights is underlined. Separations of nucleotides and DNA fragments are presented. © 1990, Taylor & Francis Group, LLC. All rights reserved.
引用
收藏
页码:319 / 330
页数:12
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