CHEMILUMINESCENCE METHOD FOR THE DETERMINATION OF ADENINE AFTER REACTION WITH PHENYLGLYOXAL

被引：20
作者：
KURODA, N
NAKASHIMA, K
AKIYAMA, S
机构：
[1] School of Pharmaceutical Sciences, Nagasaki University, Nagasaki, 852, Bunkyo-machi
来源：
ANALYTICA CHIMICA ACTA | 1993年 / 278卷 / 02期
关键词：
CHEMILUMINESCENCE; ADENINE; ADENOSINE; NUCLEIC ACID BASES;
D O I：
10.1016/0003-2670(93)85110-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A chemiluminescence (CL) method is described for the determination of adenine. Treatment of adenine with phenylglyoxal in acidic propan-2-ol gave products that produced CL in the presence of hydrogen peroxide, 2-mercaptoethanol and sodium hydroxide. The proposed method is highly sensitive and selective to adenine and adenosine. Adenine can be determined in the range 1.5 x 10(-4)-5.0 x 10(-7) M. The detection limit for adenine is 1.9 x 10(-7) M (19 pmol per assay).
引用
收藏
页码：275 / 278
页数：4
相关论文
共 9 条
[1]   ON THE REACTION OF PHENYLGLYOXAL WITH 2-AMINOPYRIDINE [J].
ALCAIDE, B ;
PEREZOSSORIO, R ;
PLUMET, J ;
SIERRA, MA .
TETRAHEDRON LETTERS, 1986, 27 (14) :1627-1630
[2]   REACTION OF GLYOXAL WITH NUCLEIC ACID COMPONENTS .3. KINETICS OF REACTION WITH MONOMERS [J].
BROUDE, NE ;
BUDOWSKY, EI .
BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 254 (03) :380-&
[3]  
GOTO T, 1968, TETRAHEDRON LETT, P3873
[4]   DETERMINATION OF ALPHA-KETO ACIDS INCLUDING PHENYLPYRUVIC ACID IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CHEMILUMINESCENCE DETECTION [J].
NAKAHARA, T ;
ISHIDA, J ;
YAMAGUCHI, M ;
NAKAMURA, M .
ANALYTICAL BIOCHEMISTRY, 1990, 190 (02) :309-313
[5]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY CHEMILUMINESCENCE DETERMINATION OF METHAMPHETAMINE IN HUMAN SERUM USING N-(4-AMINOBUTYL)-N-ETHYLISOLUMINOL AS A CHEMILUMINOGEN [J].
NAKASHIMA, K ;
SUETSUGU, K ;
AKIYAMA, S ;
YOSHIDA, M .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 530 (01) :154-159
[6]   REACTIONS WITH PURINES AND PYRIMIDINES [J].
SHAPIRO, R .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1969, 163 (A2) :624-&
[7]   SYNTHESIS OF CYPRIDINA LUCIFERIN AND RELATED COMPOUNDS .6. SYNTHESIS OF 3-HYDROXYIMIDAZO(1,2-ALPHA)PYRIDINES [J].
SUGIURA, S ;
KAKOI, H ;
INOUE, S ;
GOTO, T .
YAKUGAKU ZASSHI, 1970, 90 (04) :436-&
[8]  
YUKI H, 1988, CHEM PHARM BULL, V36, P1905
[9]   OPTIMAL LABELING OF PROTEINS WITH ACRIDINIUM ESTER [J].
ZOMER, G ;
VANDENBERG, RH ;
JANSEN, EHJM .
ANALYTICA CHIMICA ACTA, 1988, 205 (1-2) :267-271
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