ARACHIDONIC-ACID EPOXYGENASE - STEREOCHEMICAL ANALYSIS OF THE ENDOGENOUS EPOXYEICOSATRIENOIC ACIDS OF HUMAN KIDNEY CORTEX

被引：69

作者：

KARARA, A

DISHMAN, E

JACOBSON, H

FALCK, JR

CAPDEVILA, JH

机构：

[1] VANDERBILT UNIV,MED CTR,SCH MED,DIV NEPHROL,MED CTR N S-3223,NASHVILLE,TN 37232

[2] VANDERBILT UNIV,MED CTR,SCH MED,DEPT BIOCHEM,NASHVILLE,TN 37232

[3] UNIV TEXAS,SW MED CTR,DEPT MOLEC GENET,DALLAS,TX 75235

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Arachidonic acid; Cytochrome P-450; Epoxyeicosatrienoic acid; Epoxygenase;

D O I：

10.1016/0014-5793(90)81014-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass spectral and Chromatographic analysis demonstrates the presence of 14,15-, 11,12- and 8,9-epoxyeicosatrienoic acids (44%, 33% and 23% of the total, respectively) in human kidney cortex. Chiral analysis of the human renal expoxyeicosatrienoic acids shows the formation of 8,9-, 11,12- and 14,15-epoxyeicosatrienoic acids in a 1:1, 4:1 and 2:1 ratio of antipodes, respectively. These results demonstrate the biosynthetic origin of the human kidney 11,12- and 14,15-epoxyeicosatrienoic acids and suggest a role for renal cytochrome P-450 in the bioactivation of endogenous pools of arachidonic acid. © 1990.

引用

页码：227 / 230

页数：4

共 19 条

[1]

CAPDEILA JH, 1990, IN PRESS J BIOL CHEM

[2]

CAPDEVILA JH, 1990, IN PRESS METHODS ENZ

[3] NOVEL EPOXIDES FORMED DURING THE LIVER CYTOCHROME-P-450 OXIDATION OF ARACHIDONIC-ACID [J].