CRYSTAL-STRUCTURE OF DEFENSIN HNP-3, AN AMPHIPHILIC DIMER - MECHANISMS OF MEMBRANE PERMEABILIZATION

被引：444

作者：

HILL, CP

YEE, J

SELSTED, ME

EISENBERG, D

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOCHEM,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,DEPT PATHOL,LOS ANGELES,CA 90024

[3] UNIV CALIF LOS ANGELES,DEPT CHEM,LOS ANGELES,CA 90024

[4] UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024

来源：

SCIENCE | 1991年 / 251卷 / 5000期

关键词：

D O I：

10.1126/science.2006422

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Defensins (molecular weight 3500 to 4000) act in the mammalian immune response by permeabilizing the plasma membranes of a broad spectrum of target organisms, including bacteria, fungi, and enveloped viruses. The high-resolution crystal structure of defensin HNP-3 (1.9 angstrom resolution, R factor 0.19) reveals a dimeric beta-sheet that has an architecture very different from other lytic peptides. The dimeric assembly suggests mechanisms by which defensins might bind to and permeabilize the lipid bilayer.

引用

收藏

页码：1481 / 1485

页数：5

相关论文

共 50 条

[1] TWO-DIMENSIONAL NMR-STUDIES OF THE ANTIMICROBIAL PEPTIDE NP-5 [J].

BACH, AC ;

SELSTED, ME ;

PARDI, A .

BIOCHEMISTRY, 1987, 26 (14) :4389-4397

[2] CONFORMATION OF TWISTED BETA-PLEATED SHEETS IN PROTEINS [J].

CHOTHIA, C .

JOURNAL OF MOLECULAR BIOLOGY, 1973, 75 (02) :295-302

[3] DIRECT INACTIVATION OF VIRUSES BY HUMAN GRANULOCYTE DEFENSINS [J].

DAHER, KA ;

SELSTED, ME ;

LEHRER, RI .

JOURNAL OF VIROLOGY, 1986, 60 (03) :1068-1074

[4] INTERACTION OF BEE MELITTIN WITH LIPID BILAYER MEMBRANES [J].

DAWSON, CR ;

DRAKE, AF ;

HELLIWELL, J ;

HIDER, RC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 510 (01) :75-86

[5] PURIFICATION AND ANTIMICROBIAL PROPERTIES OF 3 DEFENSINS FROM RAT NEUTROPHILS [J].

EISENHAUER, PB ;

HARWIG, SSL ;

SZKLAREK, D ;

GANZ, T ;

SELSTED, ME ;

LEHRER, RI .

INFECTION AND IMMUNITY, 1989, 57 (07) :2021-2027

[6] ORTHORHOMBIC CRYSTALS AND 3-DIMENSIONAL STRUCTURE OF THE POTENT TOXIN-II FROM THE SCORPION ANDROCTONUS-AUSTRALIS HECTOR [J].

FONTECILLACAMPS, JC ;

HABERSETZERROCHAT, C ;

ROCHAT, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (20) :7443-7447

[7] ARCHITECTURE OF SCORPION NEUROTOXINS - A CLASS OF MEMBRANE-BINDING PROTEINS [J].

FONTECILLACAMPS, JC ;

ALMASSY, RJ ;

EALICK, SE ;

SUDDATH, FL ;

WATT, DD ;

FELDMANN, RJ ;

BUGG, CE .

TRENDS IN BIOCHEMICAL SCIENCES, 1981, 6 (11) :291-296

[8] A VOLTAGE-GATED ION CHANNEL MODEL INFERRED FROM THE CRYSTAL-STRUCTURE OF ALAMETHICIN AT 1.5-A RESOLUTION [J].

FOX, RO ;

RICHARDS, FM .

NATURE, 1982, 300 (5890) :325-330

[9] DEFENSINS - NATURAL PEPTIDE ANTIBIOTICS OF HUMAN-NEUTROPHILS [J].

GANZ, T ;

SELSTED, ME ;

SZKLAREK, D ;

HARWIG, SSL ;

DAHER, K ;

BAINTON, DF ;

LEHRER, RI .

JOURNAL OF CLINICAL INVESTIGATION, 1985, 76 (04) :1427-1435

[10] DEFENSINS MEDIATE THE MICROBICIDAL ACTIVITY OF HUMAN NEUTROPHIL GRANULE EXTRACT AGAINST ACINETOBACTER-CALCOACETICUS [J].

GREENWALD, GI ;

GANZ, T .

INFECTION AND IMMUNITY, 1987, 55 (06) :1365-1368

← 1 2 3 4 5 →