EVOLUTIONARY CONSERVATION OF COMPONENTS OF THE PROTEIN TRANSLOCATION COMPLEX

被引:227
作者
HARTMANN, E
SOMMER, T
PREHN, S
GORLICH, D
JENTSCH, S
RAPOPORT, TA
机构
[1] MAX DELBRUCK CTR MOLEC MED, D-12135 BERLIN, GERMANY
[2] HUMBOLDT UNIV BERLIN, INST BIOCHEM, D-10115 BERLIN, GERMANY
[3] UNIV HEIDELBERG, CTR MOLEC BIOL, ZMBH, D-69120 HEIDELBERG, GERMANY
关键词
D O I
10.1038/367654a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
PROTEIN translocation into the mammalian endoplasmic reticulum requires the Sec61p complex, which consists of three membrane proteins(1). The alpha-subunit, the homologue of Sec61p of yeast(2-4), shows some similarity to SecYp(5), a key component of the protein export apparatus of bacteria(6,7). In Escherichia coli, SecYp is also associated with two other proteins (SecEp and band-1 protein)(8,9). We have now determined the sequences of the beta- and gamma-subunits of the mammalian Sec61p complex. Sec61-gamma is homologous to SSS1p, a suppressor of sec61 mutants in Saccharomyces cerevisiae, and can functionally replace it in yeast cells. Moreover, Sec61-gamma and SSSIp are structurally related to SecEp of E. coli and to putative homologues in various other bacteria. At least two subunits of the Sec61/SecYp complex therefore seem to be keg components of the protein translocation apparatus in all classes of organisms.
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页码:654 / 657
页数:4
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