FLUORESCENT-ANTIBODY TEST FOR DETECTION OF RABIES VIRUS IN BRAIN SMEARS OF CVS-INFECTED MICE AT DIFFERENT STAGES OF DECOMPOSITION

被引：3

作者：

VALENTINI, EJG ^{[1
]}

ALBAS, A ^{[1
]}

AUGUSTO, VLM ^{[1
]}

ITO, FH ^{[1
]}

机构：

[1] UNIV SAO PAULO,FAC MED VET & ZOOTECNIA,BR-05340 SAO PAULO,BRAZIL

来源：

REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO | 1991年 / 33卷 / 03期

关键词：

D O I：

10.1590/S0036-46651991000300003

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The efficiency of the fluorescent antibody (FA) test in detecting rabies virus antigen in decomposed specimens was evaluated in simulated conditions of the safety test recommended for the assessment of residual virus in inactivated rabies vaccines. The CVS-infected mice were submitted to different treatments combining time and temperature in order to cause different stages of carcass decomposition and, the FA test was carried out sequentially at pre-determined time intervals. For the materials stored at 25-degrees-C, greater difficulties for prompt recognition of the inclusion bodies were found after 12 - 18h, whilst the specimens maintained at 4-degrees-C, the inclusions were easily visualized for up to 48h. Brain smears of carcasses kept at -20-degrees-C were suitable for adequate identification after 720 h of storage.

引用

页码：181 / 186

页数：6

共 13 条

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