THE EFFECT OF HYDROXYL RADICAL ON THE ANTIGENICITY OF NATIVE DNA

被引：48

作者：

ALAM, K ^{[1
]}

ALI, A ^{[1
]}

ALI, R ^{[1
]}

机构：

[1] AMU,FAC MED,JN MED COLL,DEPT BIOCHEM,ALIGARH 202002,INDIA

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

DNA; HYDROXYL RADICAL; ANTIBODY; SLE; B-CONFORMATION;

D O I：

10.1016/0014-5793(93)80038-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydroxyl free-radical mediated in vitro modification of native calf thymus DNA showed single-strand breaks, decrease in melting temperature and structural alteration of purine and pyrimidine bases. Experimentally induced antibodies against modified DNA exhibited polyspecificity. Native DNA, RNA and synthetic polynucleotides in B-conformation were found to be an effective inhibitor of induced antibody-immunogen interaction. Naturally occurring human anti-DNA autoantibodies showed enhanced recognition of modified DNA as compared to native polymer. IgG isolated from anti-DNA antibody-positve SLE sera by Protein A-Sepharose 4B chromatography were affinity purified on nDNA-(polylysyl-Sepharose 4B) matrix. In competitive binding experiments, the affinity-isolated IgG showed almost equal recognition of both native DNA and hydroxyl radical modified DNA. The possible role of hydroxyl radicals in the etiopathogenesis of SLE has been discussed.

引用

页码：66 / 70

页数：5

共 30 条

[1]

ALAM K, 1992, BIOCHEM INT, V26, P597

[2] NATURALLY-OCCURRING SLE ANTI-DNA ANTIBODIES RECOGNIZE UNIQUE CONFORMATION ON DNA-LYSINE PHOTOADDUCT [J].

ALAM, K ;

ISLAM, N ;

HASAN, R ;

ALI, A ;

ALI, R .

MICROBIOLOGY AND IMMUNOLOGY, 1992, 36 (09) :1003-1007

[3] BINDING OF MONOCLONAL ANTI-NATIVE DNA AUTOANTIBODIES TO DNA OF VARYING SIZE AND CONFORMATION [J].

ALI, R ;

DERSIMONIAN, H ;

STOLLAR, BD .

MOLECULAR IMMUNOLOGY, 1985, 22 (12) :1415-1422

[4] STRUCTURAL DAMAGE TO LYMPHOCYTE NUCLEI BY H2O2 OR GAMMA-IRRADIATION IS DEPENDENT ON THE MECHANISM OF OH RADICAL PRODUCTION [J].

ALLAN, IM ;

VAUGHAN, ATM ;

MILNER, AE ;

LUNEC, J ;

BACON, PA .

BRITISH JOURNAL OF CANCER, 1988, 58 (01) :34-37

[5] ANTIBODIES AGAINST FREE-RADICAL MODIFIED NATIVE DNA RECOGNIZE B-CONFORMATION [J].

ARA, J ;

ALI, A ;

ALI, R .

IMMUNOLOGICAL INVESTIGATIONS, 1992, 21 (06) :553-563

[6]

BABIOR BM, 1984, BLOOD, V64, P959

[7] HYDROGEN PEROXIDE-INDUCED BASE DAMAGE IN DEOXYRIBONUCLEIC-ACID [J].

BLAKELY, WF ;

FUCIARELLI, AF ;

WEGHER, BJ ;

DIZDAROGLU, M .

RADIATION RESEARCH, 1990, 121 (03) :338-343

[8] REACTIVE OXYGEN SPECIES INDUCE ANTIGENIC CHANGES IN DNA [J].

BLOUNT, S ;

GRIFFITHS, HR ;

LUNEC, J .

FEBS LETTERS, 1989, 245 (1-2) :100-104

[9]

BLOUNT S, 1990, CLIN EXP IMMUNOL, V81, P384

[10]

BULKLEY GB, 1983, SURGERY, V94, P407

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