INCREASED RENAL TUBULAR NA-K-ATPASE ACTIVITY IN MILAN HYPERTENSIVE RATS IN THE PREHYPERTENSIVE PERIOD

被引：23

作者：

MELZI, ML

SYREN, ML

ASSAEL, BM

SERENI, F

APERIA, A

机构：

[1] Department of Paediatrics, University of Milan, Milan, 20122

[2] Department of Paediatrics, Karolinska Institute, Stockholm

来源：

PEDIATRIC NEPHROLOGY | 1991年 / 5卷 / 06期

关键词：

MILAN HYPERTENSIVE RATS; THICK ASCENDING LIMB OF HENLE; NA-K-ATPASE ACTIVITY; DEVELOPMENT;

D O I：

10.1007/BF00857877

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

Milan hypertensive (MSH) rats develop hypertension around the 3rd-4th week of life and exhibit increased Na-pump activity in adulthood. The present study was performed to evaluate whether or not hypertension is preceded by an increase in Na-K-ATPase activity. Total and ouabain-sensitive ATPase activities were studied in single microdissected medullary thick ascending limb of Henle (mTAL) tubules from MHS, Milan normotensive (MNS) and Sprague-Dawley (SD) rats at 22-24, 26-28 and 45-60 days of age. Data are given as mean +/- SEM. Total and Na-K-ATPase activity exhibited a developmental pattern in MHS, MNS and SD rats. At 22-24 days no difference was seen between MHS and MNS animals. At 26-28 days MHS had a higher total and Na-K-ATPase activity than MNS (3031 + 171 vs 2471 + 178 pmol phosphate/mm tubule per hour, P < 0.05; 2289 + 205 vs 1653 + 151, n = 10, P < 0.05). At this age there was still no difference in mean arterial blood pressure (88 + 4 vs 86 + 3 mm Hg, n = 15). Adult MHS rats had higher blood pressure (140 + 9 vs 112 + 8 mm Hg, P < 0.001) and higher total (3544 + 136 vs 2718 + 215 pmol phosphate/mm tubule per hour, n = 10, P < 0.01) and Na-K-ATPase activity (2670 + 99 vs 1942 + 217 pmol phosphate/mm tubule per hour, n = 10, P < 0.05) than adult MNS rats. We conclude that increased Na-K-ATPase activity in mTAL precedes the development of hypertension in the Milan strain of rats.

引用

页码：700 / 703

页数：4

共 25 条

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