STRUCTURAL ORGANIZATION OF STR 246C AND STR 246N, PLANT DEFENSE-RELATED GENES FROM NICOTIANA-TABACUM

被引:10
|
作者
FROISSARD, D
GOUGH, C
CZERNIC, P
SCHNEIDER, M
TOPPAN, A
ROBY, D
MARCO, Y
机构
[1] INRA,CNRS,BIOL MOLEC RELAT PLANTES MICROORGANISMS LAB,F-31326 CASTANET TOLOSAN,FRANCE
[2] LAB BOT,F-31400 TOULOUSE,FRANCE
[3] SANOFI ELF BIORECH,LABEGE INNOPOLE,F-31328 LABEGE,FRANCE
关键词
AUXIN-INDUCIBLE GENES; DEFENSE GENES; NICOTIANA TABACUM; PSEUDOMONAS SOLANACEARUM; REPEATED DNA SEQUENCES; INTEGRASE;
D O I
10.1007/BF00039563
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously identified a cDNA clone, pNt246, whose corresponding transcripts accumulate in leaves in response to inoculation by compatible and incompatible isolates of the phytopathogenic bacterium Pseudomonas solanacearum [19]. We now describe the nucleotide sequence of a genomic clone, str 246C, corresponding to this cDNA species, and of a related genomic clone, sfr 246N, which appears to be a pseudogene with a 5'-end deletion. The nucleotide sequence of the str 246C gene was found to be identical to that of the parA gene, previously shown to be regulated by auxin [28, 29]. Upstream of the str 246N gene, sequences homologous to a Barn HI repetitive element described in Vicia faba [15] are present within an ORF showing significant homologies to an integrase-encoding gene of several retroviruses. This observation indicates that this highly repetitive DNA originates from sequences present in transposable mobile elements.
引用
收藏
页码:515 / 521
页数:7
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