PHOTOAFFINITY-LABELING OF THE PHYLLOQUINONE-BINDING POLYPEPTIDES BY 2-AZIDOANTHRAQUINONE IN PHOTOSYSTEM-I PARTICLES

被引：8

作者：

IWAKI, M

TAKAHASHI, M

SHIMADA, K

TAKAHASHI, Y

ITOH, S

机构：

[1] KONAN UNIV, FAC SCI, DEPT BIOL, KOBE 658, JAPAN

[2] TOKYO METROPOLITAN UNIV, FAC SCI, DEPT BIOL, MACHIOJI, TOKYO 192031, JAPAN

[3] OKAYAMA UNIV, FAC SCI, DEPT BIOL, OKAYAMA 700, JAPAN

[4] OKAYAMA UNIV, GRAD SCH SCI & TECHNOL, OKAYAMA 700, JAPAN

来源：

FEBS LETTERS | 1992年 / 312卷 / 01期

关键词：

PHOTOSYSTEM-I; REACTION CENTER; PHOTOAFFINITY LABEL; PHYLLOQUINONE (VITAMIN-K1); ELECTRON TRANSFER;

D O I：

10.1016/0014-5793(92)81403-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A photoaffinity label, 2-azido-9,10-anthraquinone, binds at the quinone-binding (Q(phi)) site with high affinity and can substitute for the secondary acceptor, phylloquinone, in photosystem I reaction center of spinach. Phylloquinone-depleted photosystem I particles reconstituted with azido-[H-3]anthraquinone were illuminated with UV light and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The large core polypeptides (psaA and/or psaB) were selectively labeled. The labeling was competitively inhibited in the presence of anthraquinone. These results indicate that the Q(phi) site is located on psaA or psaB polypeptides.

引用

页码：27 / 30

页数：4

共 21 条

[1] STRUCTURE OF THE REACTION CENTER FROM RHODOBACTER-SPHAEROIDES R-26 - THE PROTEIN SUBUNITS [J].