RECOMBINATION-DEFICIENT MUTANTS OF SALMONELLA-TYPHIMURIUM ARE AVIRULENT AND SENSITIVE TO THE OXIDATIVE BURST OF MACROPHAGES

被引：149

作者：

BUCHMEIER, NA

LIPPS, CJ

SO, MYH

HEFFRON, F

机构：

[1] OREGON HLTH SCI UNIV,DEPT MICROBIOL & IMMUNOL,PORTLAND,OR 97201

[2] UNIV CALIF SAN DIEGO,DEPT PATHOL,SAN DIEGO,CA 92103

来源：

MOLECULAR MICROBIOLOGY | 1993年 / 7卷 / 06期

关键词：

D O I：

10.1111/j.1365-2958.1993.tb01184.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage-like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative burst of phagocytes is one source of such DNA damage.

引用

页码：933 / 936

页数：4

共 19 条

[1]

BEAMAN L, 1984, ANNU REV MICROBIOL, V38, P27

[2]

BUCHMEIER NA, 1989, INFECT IMMUN, V57, P1

[3] POSITIVE CONTROL OF A REGULON FOR DEFENSES AGAINST OXIDATIVE STRESS AND SOME HEAT-SHOCK PROTEINS IN SALMONELLA-TYPHIMURIUM [J].