DIFFERENTIAL EXPRESSION OF SEVERAL E2-TYPE UBIQUITIN CARRIER PROTEIN GENES AT DIFFERENT DEVELOPMENTAL STAGES IN ARABIDOPSIS-THALIANA AND NICOTIANA-SYLVESTRIS

被引：34

作者：

GENSCHIK, P

DURR, A

FLECK, J

机构：

[1] Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg-Cedex, 67084, 12, rue du Général Zimmer

来源：

MOLECULAR AND GENERAL GENETICS | 1994年 / 244卷 / 05期

关键词：

PLANTS; STRESS; CELL CYCLE; GENE EXPRESSION; UBIQUITIN CARRIER PROTEINS;

D O I：

10.1007/BF00583906

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We characterized three genes encoding different E2-type ubiquitin carrier proteins involved in the ubiquitin-mediated proteolytic pathway: UbcAt3 shows homologies to the yeast CDC34 gene and UbcAt4a and UbcAt4b are two different genes homologous to the Ubc1/4/5 subfamily in yeast. Their accumulation was analysed and compared with that of the different families encoding polyubiquitins, as well as the monoubiquitin fusion protein, which is considered as a marker for cell division, during various developmental stages including G0/S transition and senescence of higher plant cells. Our results imply that these Ubc genes are under the control of complex mechanisms, and are differentially regulated, but not necessarily co-regulated with ubiquitin genes. Even the closely related UbcAt4a and UbcAt4b genes of the same multigene subfamily are controlled by distinct regulatory mechanisms.

引用

页码：548 / 556

页数：9

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