MATHEMATICAL-MODEL FOR TIME-RESOLVED AND FREQUENCY-DOMAIN FLUORESCENCE SPECTROSCOPY IN BIOLOGICAL TISSUE

被引：219

作者：

PATTERSON, MS

POGUE, BW

机构：

[1] MCMASTER UNIV, HAMILTON L8U 5C2, ON, CANADA

[2] ONTARIO LASER & LIGHTWAVE RES CTR, TORONTO, ON, CANADA

来源：

APPLIED OPTICS | 1994年 / 33卷 / 10期

关键词：

FLUORESCENCE SPECTROSCOPY; TISSUE OPTICS; DIFFUSION THEORY; MULTIPLE SCATTERING;

D O I：

10.1364/AO.33.001963

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

In general it is not possible to write an analytic expression for the fluorescence signal generated by a fluorophore distributed in a scattering medium such as tissue. However, by assuming that the scattering properties of the tissue are the same at the excitation and emission wavelengths, we have derived a simple relation between the fluorescence and the scatter signals. Along with diffusion theory, this was used to write expressions for the fluorescence signal detected at the tissue surface in both the time and the frequency domains. Experiments using the fluorophore aluminum chlorosulfonated phthalocyanine in tissue-simulating materials confirmed the accuracy of the model. Applications to in vivo spectroscopy are discussed.

引用

页码：1963 / 1974

页数：12

共 27 条

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