A SEQUENCE ELEMENT ASSOCIATED WITH THE PLASMODIUM-FALCIPARUM KAHRP GENE IS THE SITE OF DEVELOPMENTALLY REGULATED PROTEIN-DNA INTERACTIONS

被引：51
作者：
LANZER, M [1 ]
DEBRUIN, D [1 ]
RAVETCH, JV [1 ]
机构：
[1] SLOAN KETTERING MEM CANC CTR, DIV MOLEC BIOL, DEWITT WALLACE RES LAB, 1275 YORK AVE, NEW YORK, NY 10021 USA
来源：
NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 12期
关键词：
D O I：
10.1093/nar/20.12.3051
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Plasmodium falciparum gene encoding the knob associated histidine-rich protein (KAHRP) is shown to be transcriptionally regulated during its expression in the intraerythrocytic cycle as demonstrated by stage specific nuclear run-on analysis. The genomic organization of the KAHRP gene was determined and the structural basis for the stage specific transcription investigated. A sequence motif with two-fold symmetry was found 160 bp upstream of the RNA initiation site. This sequence element interacts with parasite derived nuclear extracts in a stage specific manner that correlates with the transcriptional activity of the KAHRP gene. These studies suggest a functional role for this structural element in the developmental regulation of a P. falciparum erythrocytic gene.
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页码：3051 / 3056
页数：6
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