DENITRIFICATION ENZYMES OF BACILLUS-STEAROTHERMOPHILUS

被引：4

作者：

HO, TP ^{[1
]}

JONES, AM ^{[1
]}

HOLLOCHER, TC ^{[1
]}

机构：

[1] BRANDEIS UNIV,DEPT BIOCHEM,WALTHAM,MA 02254

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 114卷 / 02期

基金：

美国国家科学基金会;

关键词：

BACILLUS-STEAROTHERMOPHILUS; NITRATE REDUCTASE; NITRITE REDUCTASE; NITRIC OXIDE REDUCTASE; NITROUS OXIDE REDUCTASE; THERMOPHILIC DENITRIFICATION;

D O I：

10.1111/j.1574-6968.1993.tb06563.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The reductases of the denitrification pathway of Bacillus stearothermophilus, a thermophilic denitrifier, were surveyed in vitro. Nitrate, nitrite and nitric oxide reductases were found to be membrane-bound, and nitrite reductase may be a copper-containing enzyme by virtue of its rapid inactivation by the chelator, diethyldithiocarbamate. Nitric oxide reductase exhibited an optimal rate at about pH 7 rather than 5.0-5.5, as expected from the enzyme utilized by several mesophiles. Nitrous oxide reductase was not detected in assays employing reduced benzyl or methyl viologen, in spite of the appreciable N2O-uptake activity of intact cells.

引用

页码：135 / 138

页数：4

共 29 条

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