NEW MILK MEDIUM FOR GERM TUBE AND CHLAMYDOCONIDIA PRODUCTION BY CANDIDA-ALBICANS

被引：20
作者：
JITSURONG, S
KIAMSIRI, SP
PATTARARANGRONG, N
机构：
[1] PRINCE SONGKLA UNIV,FAC SCI,DEPT BIOL,SONGKHLA,THAILAND
[2] PRINCE SONGKLA UNIV,FAC SCI,DEPT MICROBIOL,SONGKHLA,THAILAND
来源：
MYCOPATHOLOGIA | 1993年 / 123卷 / 02期
关键词：
CANDIDA-ALBICANS; CHLAMYDOCONIDIA; GERM TUBE;
D O I：
10.1007/BF01365086
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A new medium consisting of UHT milk, tween 80 and agar is described for the development of both germ tube and chlamydoconidia by Candida albicans. In total 172 isolates from clinical specimens, including C. albicans (112), C. guilliermondii (4), C. krusei (3), C. parasilopsis (16). C. tropicalis (28), Torulopsis glabrata (6) and Trichosporon beigellii (3), were examined in this medium by using the standard method. A higher percentage (98.2%) of germ tube production by C. albicans was found in this medium than in undiluted serum (90.2%). In addition, only C. albicans was found to be able to produce a high percentage of chlamydoconidia (95.5%) after 48 hours' incubation. In comparison with the conventional medium, corn meal tween 80 agar (21.4%), this new medium gives a significantly higher percentage and abundance of chlamydoconidia production. Being simple, cheap and easy to prepare, the new milk medium is proposed as very practical in the clinical mycology laboratory.
引用
收藏
页码：95 / 98
页数：4
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