IMMUNOGOLD-SILVER STAINING OF IMMUNE DEPOSITS IN RENAL BIOPSIES

被引:4
作者
MCKINNON, AD
机构
[1] University of Aberdeen, Department of Pathology, University Medical Buildings, Foresterhill, Aberdeen
关键词
ELECTRON MICROSCOPY; IMMUNE DEPOSITS; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD; LIGHT MICROSCOPY; LR WHITE ACRYLIC RESIN; RENAL BIOPSIES;
D O I
10.1179/his.1993.16.3.243
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A procedure that facilitates the light and electron microscopic demonstration of immune deposits in renal biopsies with immunogold staining is described. Examination of a single specimen is achieved with both light and electron microscopy when LR White acrylic resin is used. Minimal aldehyde fixation and low temperature processing retains maximum antigen availability. For light microscopy, an indirect immunogold silver system is applied to 2 mum LR White sections, which reveal immunoglobulins and complement C3 in the familiar pattern associated with various glomerular diseases. Indirect immunogold is applied to ultrathin sections prepared from the same block to demonstrate labeling of the same antigens at the ultrastructural level.
引用
收藏
页码:243 / 247
页数:5
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