MUSCARINIC AND BETA-ADRENERGIC DEPRESSION OF THE SLOW CA-2+-ACTIVATED POTASSIUM CONDUCTANCE IN HIPPOCAMPAL CA3 PYRAMIDAL CELLS IS NOT MEDIATED BY A REDUCTION OF DEPOLARIZATION-INDUCED CYTOSOLIC CA-2+-TRANSIENTS

被引：93

作者：

KNOPFEL, T ^{[1
]}

VRANESIC, I ^{[1
]}

GAHWILER, BH ^{[1
]}

BROWN, DA ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,DEPT PHARMACOL,LONDON WC1E 6BT,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 11期

关键词：

afterhyperpolarization; fura-2; voltage clamp;

D O I：

10.1073/pnas.87.11.4083

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Combined intracellular and microfluorometric recording techniques were used to evaluate whether the inhibition by cholinergic or adrenergic transmitters of the Ca2+-activated potassium current (I(AHP)) in hippocampal CA3 pyramidal cells was mediated by an alteration of depolarization-induced change in cytosolic free Ca2+ concentration ([Ca2+](i)). Low concentrations of isoproterenol (1-10 μM) and muscarine (0.25-1 μM) reversibly abolished I(AHP) without affecting concomitant Ca2+ transients or the steady-state [Ca2+][(i). Only after application of higher concentrations of muscarine, [Ca2+](i) increased; in the presence of potassium channel blockers, muscarine depressed Ca2+ currents and concomitant Ca2+ transients. These observations provide direct evidence that the inhibition of I(AHP) by isoproterenol and muscarine are not mediated by an alteration of Ca2+ dynamics.

引用

页码：4083 / 4087

页数：5

共 38 条

[1] EPILEPTIFORM BURST AFTERHYPERPOLARIZATION - CALCIUM-DEPENDENT POTASSIUM POTENTIAL IN HIPPOCAMPAL CA1-PYRAMIDAL CELLS [J].

ALGER, BE ;

NICOLL, RA .

SCIENCE, 1980, 210 (4474) :1122-1124

[2] PROTEIN KINASE-C REGULATES IONIC CONDUCTANCE IN HIPPOCAMPAL PYRAMIDAL NEURONS - ELECTROPHYSIOLOGICAL EFFECTS OF PHORBOL ESTERS [J].

BARABAN, JM ;

SNYDER, SH ;

ALGER, BE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (08) :2538-2542

[3] IONIC MECHANISMS OF CHOLINERGIC EXCITATION IN MAMMALIAN HIPPOCAMPAL PYRAMIDAL CELLS [J].

BENARDO, LS ;

PRINCE, DA .

BRAIN RESEARCH, 1982, 249 (02) :333-344

[4] AN ANALYSIS OF THE DEPOLARIZATION PRODUCED IN GUINEA-PIG HIPPOCAMPUS BY CHOLINERGIC RECEPTOR STIMULATION [J].

BENSON, DM ;

BLITZER, RD ;

LANDAU, EM .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 404 :479-496

[5] MUSCARINIC CHOLINERGIC STIMULATION INCREASES CYCLIC-GMP LEVELS IN RAT HIPPOCAMPUS [J].

BLACK, AC ;

SANDQUIST, D ;

WEST, JR ;

WAMSLEY, JK ;

WILLIAMS, TH .

JOURNAL OF NEUROCHEMISTRY, 1979, 33 (06) :1165-1168

[6]

BUCKLEY NJ, 1988, J NEUROSCI, V8, P4646

[7] THE PHARMACOLOGY OF CHOLINERGIC EXCITATORY RESPONSES IN HIPPOCAMPAL PYRAMIDAL CELLS [J].

COLE, AE ;

NICOLL, RA .

BRAIN RESEARCH, 1984, 305 (02) :283-290

[8] ACETYLCHOLINE MEDIATES A SLOW SYNAPTIC POTENTIAL IN HIPPOCAMPAL PYRAMIDAL CELLS [J].

COLE, AE ;

NICOLL, RA .

SCIENCE, 1983, 221 (4617) :1299-1301

[9] CHARACTERIZATION OF A SLOW CHOLINERGIC POST-SYNAPTIC POTENTIAL RECORDED INVITRO FROM RAT HIPPOCAMPAL PYRAMIDAL CELLS [J].

COLE, AE ;

NICOLL, RA .

JOURNAL OF PHYSIOLOGY-LONDON, 1984, 352 (JUL) :173-188

[10] CYCLIC-GMP DEPRESSES HIPPOCAMPAL CA-2+ CURRENT THROUGH A MECHANISM INDEPENDENT OF CGMP-DEPENDENT PROTEIN-KINASE [J].

DOERNER, D ;

ALGER, BE .

NEURON, 1988, 1 (08) :693-699

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