MEASUREMENT OF LEUKOCYTE MOTILITY AND CHEMOTAXIS PARAMETERS USING A QUANTITATIVE-ANALYSIS OF THE UNDER-AGAROSE MIGRATION ASSAY

The correlation between leukocyte motility properties and the effective function of the inflammatory defense response has stimulated efforts to characterize random motility and chemotaxis using in vitro assays. In order that in vitro measurements may be useful in predicting in vivo behavior, the experimental data must be used to determine quantitative parameters upon which the in vivo results will depend. The key parameters for leukocyte migration are the random motility coefficient and chemotactic coefficient. In this study, the under-agarose migration assay of Nelson et al. and others is modeled and analyzed mathematically. The relationship between the experimental cell migration-distance data and the random motility and chemotactic coefficients is determined. © 1979.