Evaluation of Serum-Free, Xeno-Free Cryopreservation Solutions for Human Adipose-Derived Mesenchymal Stem Cells

被引：17

作者：

Miyagi-Shiohira, Chika ^{[1
]}

Kobayashi, Naoya ^{[2
]}

Saitoh, Issei ^{[3
]}

Watanabe, Masami ^{[4
]}

Noguchi, Yasufumi ^{[5
]}

Matsushita, Masayuki ^{[6
]}

Noguchi, Hirofumi ^{[1
]}

机构：

[1] Univ Ryukyus, Grad Sch Med, Dept Regenerat Med, 207 Uehara, Nishihara, Okinawa 9030215, Japan

[2] Okayama Saidaiji Hosp, Okayama, Japan

[3] Niigata Univ, Grad Sch Med & Dent Sci, Div Pediat Dent, Niigata, Japan

[4] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Urol, Okayama, Japan

[5] Hiroshima Int Univ, Dept Socioenvironm Design, Hiroshima, Japan

[6] Univ Ryukyus, Grad Sch Med, Dept Mol & Cellular Physiol, Nishihara, Okinawa, Japan

来源：

CELL MEDICINE | 2016年 / 9卷 / 1-2期

基金：

日本学术振兴会;

关键词：

Adipose-derived mesenchymal stem cells (ASCs); Cryopreservation; Dimethyl sulfoxide (DMSO); Cell Banker 3; TC protector;

D O I：

10.3727/215517916X693122

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into cells of mesodermal origin, such as osteoblasts, adipocytes, myocytes, and chondrocytes, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. For clinical application of ASCs, serum-free, xeno-free cryopreservation solutions should be used. This study determined the viability and adipo-osteogenic potential of cryopreserved ASCs using four cryopreservation solutions: 10% DMSO, Cell Banker 2 (serum free), Stem Cell Banker (=Cell Banker 3: serum free, xeno free), and TC protector (serum free, xeno free). The viability of the cryopreserved ASCs was over 80% with all cryopreservation solutions. No difference in the adipo-osteogenic potential was found between the cells that did or did not undergo cryopreservation in these cryopreservation solutions. These data suggest that Cell Banker 3 and TC protector are comparable with 10% DMSO and Cell Banker 2 for ASCs, and cryopreserved as well as noncryopreserved ASCs could be applied for regenerative medicine.

引用

页码：15 / 20

页数：6

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