CLONING AND PHARMACOLOGICAL CHARACTERIZATION OF A RAT MU-OPIOID RECEPTOR

被引：390

作者：

THOMPSON, RC

MANSOUR, A

AKIL, H

WATSON, SJ

机构：

[1] Mental Health Research Institute University of Michigan, Ann Arbor

来源：

NEURON | 1993年 / 11卷 / 05期

关键词：

D O I：

10.1016/0896-6273(93)90120-G

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have isolated a rat cDNA clone that displays 75% amino acid homology with the mouse delta and rat kappa opioid receptors. The cDNA (designated pRMuR-12) encodes a protein of 398 amino acids comprising, in part, seven hydrophobic domains similar to those described for other G protein-linked receptors. Data from binding assays conducted with COS-1 cells transiently transfected with a CMV mammalian expression vector containing the full coding region of pRMuR-12 demonstrated mu receptor selectivity. In situ hybridization mRNA analysis revealed an mRNA distribution in rat brain that corresponds well to the distribution of binding sites labeled with mu-selective ligands. Based upon these observations, we conclude that pRMuR-12 encodes a mu opioid receptor.

引用

页码：903 / 913

页数：11

共 60 条

[1] KAPPA-OPIATE AGONISTS INHIBIT ADENYLATE-CYCLASE AND PRODUCE HETEROLOGOUS DESENSITIZATION IN RAT SPINAL-CORD [J].