A SENSITIVE ENZYME-IMMUNOASSAY FOR THE DETECTION OF A SYNTHETIC AFFINITY LIGAND, THE REACTIVE YELLOW-13 DYE

被引：3

作者：

REGNAULT, V ^{[1
]}

VALLAR, L ^{[1
]}

RIVAT, C ^{[1
]}

STOLTZ, JF ^{[1
]}

BOSCHETTI, E ^{[1
]}

机构：

[1] SEPRACOR IBF,F-92390 VILLENEUVE GARENN,FRANCE

来源：

JOURNAL OF IMMUNOASSAY | 1992年 / 13卷 / 04期

关键词：

REACTIVE YELLOW-13; ENZYME IMMUNOASSAY; DYE-AFFINITY CHROMATOGRAPHY; ANTI-DYE ANTIBODIES;

D O I：

10.1080/15321819208019832

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dye-affinity chromatography is widely and increasingly used for the isolation of various proteins. In particular, the purification of transthyretin can be efficiently achieved by affinity chromatography on immobilized Reactive Yellow 13. Measurement of trace-amounts of dye leaching from affinity columns is important because of possible toxicity or side effects linked with the presence of dye in therapeutic transthyretin preparations. A competitive enzyme immunoassay was developed to monitor yellow-dye column leaching. Biotinylated rabbit anti-Reactive Yellow 13 antibodies (immunoglobulin G fraction) were used as principal reagent. The assay is specific, sensitive to 1 ng/ml of Reactive Yellow 13, has a good reproducibility and allows the accurate detection of the dye in the presence of transthyretin.

引用

页码：509 / 520

页数：12

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