PURIFICATION AND PROPERTIES OF LIPOXYGENASE IN MARCHANTIA-POLYMORPHA CULTURED-CELLS

被引：9
作者：
MATSUI, K
NARAHARA, H
KAJIWARA, T
HATANAKA, A
机构：
[1] Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi
来源：
PHYTOCHEMISTRY | 1991年 / 30卷 / 05期
关键词：
MARCHANTIA-POLYMORPHA; MARCHANTIACEAE; LIVERWORT; PURIFICATION; LIPOXYGENASE;
D O I：
10.1016/0031-9422(91)84196-Y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lipoxygenase activity has been detected in cultured cells of a bryophyte, Marchantia polymorpha (liverwort). The activity was enhanced in the stationary phase. Most of the activity occurred in the cytosolic fraction. The lipoxygenase was purified to homogeneity by ammonium sulphate fractionation and hydrophobic, ion-exchange, and gel filtration chromatography. From the SDS-polyacylamide gel electrophoresis, the M(r) of the lipoxygenase was estimated as 109 600. The pH optimum was ca 9.0 and the pI value was 4.8. This enzyme formed mainly 13-(S)-hydroperoxy-(9Z,11E)-octadecadienoic acid from linoleic acid. It showed twice the activity for gamma-linolenic acid than for linoleic acid.
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页码：1499 / 1502
页数：4
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