SENSITIVE NEW SUBSTRATE FOR CHYMOTRYPSIN

被引：598

作者：

DELMAR, EG

LARGMAN, C

BRODRICK, JW

GEOKAS, MC

机构：

[1] Enzymology Research, Martinez Veteran's Administration Medical Center, Martinez

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 99卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80013-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new substrate for chymotrypsin, succinyl-Ala-Ala-Pro-Phe-p-nitroanilide has been synthesized. Kinetic analysis of the hydrolysis of this peptide by bovine chymotrypsin gave a Km of 0.043 mm and akcat of 45 s-1. This substrate is readily soluble and stable to hydrolysis in Tris buffer at pH 8. Chymotrypsin levels down to 1.0 ng can be rapidly determined spectrophotometrically. © 1979 Academic Press, Inc.

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页码：316 / 320

页数：5

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