CALCIUM-VOLTAGE-DEPENDENT AND VOLTAGE-DEPENDENT POTASSIUM CHANNEL IN THE RAT RETINAL AMACRINE CELLS IDENTIFIED INVITRO USING A CELL TYPE-SPECIFIC MONOCLONAL-ANTIBODY

被引：19
作者：
AKAGAWA, K
TAKADA, M
HAYASHI, H
UYEMURA, K
机构：
[1] SAITAMA MED SCH,DEPT PHYSIOL 1,MOROYAMA,SAITAMA 35004,JAPAN
[2] SAITAMA MED SCH,DEPT PHYSIOL 2,MOROYAMA,SAITAMA 35004,JAPAN
来源：
BRAIN RESEARCH | 1990年 / 518卷 / 1-2期
关键词：
Amacrine cell in vitro; Monoclonal antibody HPC-1; Potassium channel;
D O I：
10.1016/0006-8993(90)90945-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Single-channel currents were studied from identified amacrine cells of rat retinal monolayer cultures. Cells were immunohistochemically identified using an amacrine cell-specific monoclonal antibody, HPC-1. Single-channel currents with the following properties were observed. (1) The mean opening duration was voltage dependent, tending to increase on hyperpolarization. (2) The reversal potential was almost identical to the calculated EK derived from the potassium concentration gradient. (3) The conductance was 110 pS at a low extracellular potassium concentration. (4) Currents were blocked by TEA. (5) Mean opening duration was also dependent on the calcium concentration of the intracellular side. These properties were consistent with the voltage and calcium-dependent potassium channels of a large conductance. Our results also demonstrated that cell-type-specific monoclonal antibodies together with electrophysiological recordings offer a very useful method to investigate the physiological properties of distinct neuronal types in mixed cell cultures. © 1990.
引用
收藏
页码：1 / 5
页数：5
相关论文
共 14 条
[1]  
AKAGAWA K, 1988, BIOMED RES-TOKYO, V9, P161
[2]   SELECTIVE LOCALIZATION OF GLYCINE-ACCUMULATING CELLS IN REAGGREGATE CULTURE OF RAT RETINA [J].
AKAGAWA, K ;
BARNSTABLE, CJ .
DEVELOPMENTAL BRAIN RESEARCH, 1987, 31 (01) :124-128
[3]   IDENTIFICATION AND CHARACTERIZATION OF CELL-TYPES IN MONOLAYER-CULTURES OF RAT RETINA USING MONOCLONAL-ANTIBODIES [J].
AKAGAWA, K ;
BARNSTABLE, CJ .
BRAIN RESEARCH, 1986, 383 (1-2) :110-120
[4]   HISTIOTYPIC ORGANIZATION AND CELL-DIFFERENTIATION IN RAT RETINAL REAGGREGATE CULTURES [J].
AKAGAWA, K ;
HICKS, D ;
BARNSTABLE, CJ .
BRAIN RESEARCH, 1987, 437 (02) :298-308
[5]   IDENTIFICATION AND CHARACTERIZATION OF CELL-TYPES ACCUMULATING GABA IN RAT RETINAL CULTURES USING CELL TYPE SPECIFIC MONOCLONAL-ANTIBODIES [J].
AKAGAWA, K ;
BARNSTABLE, CJ .
BRAIN RESEARCH, 1987, 408 (1-2) :154-162
[6]   MONOCLONAL-ANTIBODIES THAT LABEL DISCRETE CELL-TYPES IN THE MAMMALIAN NERVOUS-SYSTEM [J].
BARNSTABLE, CJ ;
AKAGAWA, K ;
HOFSTEIN, R ;
HORN, JP .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1983, 48 :863-876
[7]   A MARKER OF EARLY AMACRINE CELL-DEVELOPMENT IN RAT RETINA [J].
BARNSTABLE, CJ ;
HOFSTEIN, R ;
AKAGAWA, K .
DEVELOPMENTAL BRAIN RESEARCH, 1985, 20 (02) :286-290
[8]   CALCIUM-ACTIVATED POTASSIUM CHANNELS [J].
BLATZ, AL ;
MAGLEBY, KL .
TRENDS IN NEUROSCIENCES, 1987, 10 (11) :463-467
[9]   IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].
HAMILL, OP ;
MARTY, A ;
NEHER, E ;
SAKMANN, B ;
SIGWORTH, FJ .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100
[10]   CA-DEPENDENT K-CHANNELS WITH LARGE UNITARY CONDUCTANCE IN CHROMAFFIN CELL-MEMBRANES [J].
MARTY, A .
NATURE, 1981, 291 (5815) :497-500
12