NEUTRAL PROTEASES OF GENUS BACILLUS

被引:51
作者
KEAY, L
机构
[1] New Enterprise Division, Monsanto Company, St. Louis, MO
关键词
D O I
10.1016/0006-291X(69)90323-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neutral protease of B. subtilis NRRL B3411 has been highly purified by chromatography on hydroxylapatite, and compared with B. subtilis var. amylosacchariticus neutral protease and thermolysin. On the basis of activity against macromolecular substrates no differentiation was possible. Immunochemical and stability studies as well as amino acid composition distinguished thermolysin from the other proteases which were indistinguishable. © 1969.
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页码:257 / &
相关论文
共 15 条
[1]   The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin [J].
Anson, ML .
JOURNAL OF GENERAL PHYSIOLOGY, 1938, 22 (01) :79-89
[3]   STUDIES ON SPECIFICITY OF BACILLUS SUBTILIS NEUTRAL PROTEASE WITH INSULIN B-CHAIN [J].
FEDER, J ;
LEWIS, C .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 28 (03) :318-&
[4]  
FEDER J, 1968, BIOCHEM BIOPHYS RES, V32, P316
[5]   DIFFERENTIATION OF ALKALINE PROTEASES FROM BACILLUS SPECIES [J].
KEAY, L ;
MOSER, PW .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 34 (05) :600-&
[6]  
MANNING GB, 1961, J BIOL CHEM, V236, P2952
[7]  
MCCONN JD, 1964, J BIOL CHEM, V239, P3706
[9]   COMPARISON OF SPECIFICITIES OF VARIOUS NEUTRAL PROTEINASES FROM MICROORGANISMS [J].
MORIHARA, K ;
TSUZUKI, H ;
OKA, T .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 123 (03) :572-&
[10]  
OHTA Y, 1966, J BIOL CHEM, V241, P5919