Expression analysis of genes encoding TEX11, TEX12, TEX14 and TEX15 in testis tissues of men with non-obstructive azoospermia

被引:27
作者
Boroujeni, Parnaz Borjian [1 ]
Sabbaghian, Marjan [2 ]
Totonchi, Mehdi [1 ,3 ]
Sodeifi, Niloofar [2 ]
Sarkardeh, Homa [1 ]
Samadian, Azam [3 ]
Sadighi-Gilani, Mohammad Ali [2 ,4 ]
Gourabi, Hamid [1 ]
机构
[1] ACECR, Dept Genet, Reprod Biomed Res Ctr, Royan Inst Reprod Biomed, Tehran, Iran
[2] ACECR, Dept Androl, Reprod Biomed Res Ctr, Royan Inst Reprod Biomed, Tehran, Iran
[3] ACECR, Dept Stem Cells & Dev Biol, Cell Sci Res Ctr, Royan Inst Stem Cell Biol & Technol, Tehran, Iran
[4] Univ Tehran Med Sci, Dept Urol, Shariati Hosp, Tehran, Iran
来源
JORNAL BRASILEIRO DE REPRODUCAO ASSISTIDA | 2018年 / 22卷 / 03期
关键词
Male infertility; Gene expression; Non-Obstructive Azoospermia; Intercellular bridges; Synaptonemal complex;
D O I
10.5935/1518-0557.20180030
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: Spermatogenesis is a complex process controlled by a plethora of genes. Changes in expression and function of these genes may thus lead to spermatogenic deficiency and male infertility. TEX11, TEX12, TEX14 and TEX15 are germ cell-specific genes expressed in the testis. TEX11, involved in the initiation and maintenance of chromosome synapses in meiotic chromosomes, has been shown to be essential for meiosis and fertility in males. TEX14, a component of intercellular bridges in germ cells, is required for spermatogenesis and fertility. TEX12 and TEX15 are essential for correct assembly of the synaptonemal complex and thus meiosis progression. Methods: In order to examine whether changes in expression of these genes is associated with impaired spermatogenesis, expression levels of these genes were quantified by RT-qPCR on samples retrieved from infertile patients submitted to diagnostic testicular biopsy at Royan institute. Samples were divided into two groups of 18 patients with non-obstructive azoospermia considered as case; nine patients with obstructive azoospermia were included in the control group. Results: A significant down-regulation of these genes was observed in the SCOS group when compared to the control group. Conclusion: This result suggests that regular expression of TEX11, TEX12, TEX14 and TEX15 is essential for the early stages of spermatogenesis.
引用
收藏
页码:185 / 192
页数:8
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