Virulence and resistance features of Pseudomonas aeruginosa strains isolated from patients with cardiovascular diseases

被引：0

作者：

Merezeanu, Nicoleta ^{[1
]}

Gheorghe, Irina ^{[2
,3
]}

Popa, Marcela ^{[2
,3
]}

Chifiriuc, Mariana Carmen ^{[2
,3
]}

Lazar, Veronica ^{[2
,3
]}

Pantea, Octav ^{[1
]}

Banu, Otilia ^{[4
]}

Bolocan, Alexandra ^{[5
,6
]}

Grigore, Raluca ^{[5
,6
]}

Beresteanu, Serban Vifor ^{[5
,6
]}

机构：

[1] Univ Oil & Gas, Ploiesti, Romania

[2] Univ Bucharest, Fac Biol, Dept Microbiol, Bucharest, Romania

[3] Univ Bucharest, ICUB, Res Inst, Bucharest, Romania

[4] Natl Inst Cardiovasc Dis Prof CC Iliescu, Bucharest, Romania

[5] Emergency Univ Hosp, Bucharest, Romania

[6] Carol Davila Univ Med & Pharm, Bucharest, Romania

来源：

BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY | 2016年 / 6卷 / 02期

关键词：

Pseudomonas aeruginosa; virulence; resistance; genetic determinants;

D O I：

暂无

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The purpose of this study was to evaluate the resistance and virulence profiles of Pseudomonas (P) aeruginosa clinical strains isolated in 2014 from patients with cardiovascular diseases hospitalized in the National Institute for Cardiovascular Diseases Prof. C.C. lliescu, Bucharest. The phenotypic screening evaluated eight soluble virulence factors (haemolysins, lecithinase, lipase, caseinase, gelatinase, amylase. DNase. aesculin hydrolysis) and the resistance profiles have been determined by using disk diffusion and the automated Vitek II system. Seven virulence genes encoding for protease IV, three exoengymes (exoS. exoT, exoU), two phospholipases (plcH-haemolytic phospholipase C and plcN- nonhaemolytic phospholipase C) and alginate as well as the genes involved in the beta-lactams resistance were investigated by PCR. The molecular analysis through PCR showed that 38.8% of P.aeruginosa strains expressed the gene codifying for protease IV, 22.22% the ExoS gene, 16.16% the plcH and ExoT genes and 11.11% of the analyzed strains harbored the ExoU and plcN genes. Regarding the resistance genes our study revealed 22.22% of the isolates positives for imipenemase gene (bla(IMP)) which the clincal outcome of infaction is very useful for setting up efficient preventive and therapeutic procedures for hospitalized patients with cardiovascular diseases, which are colonized or infected with P. aeruginosa strains.

引用

页码：1117 / 1121

页数：5

共 20 条

[1] Pseudomonas aeruginosa: all roads lead to resistance [J].