DEVELOPMENTAL AND FUNCTIONAL IMPAIRMENT OF T-CELLS IN MICE LACKING CD3-ZETA CHAINS

被引：155

作者：

OHNO, H ^{[1
]}

AOE, T ^{[1
]}

TAKI, S ^{[1
]}

KITAMURA, D ^{[1
]}

ISHIDA, Y ^{[1
]}

RAJEWSKY, K ^{[1
]}

SAITO, T ^{[1
]}

机构：

[1] UNIV COLOGNE, INST GENET, D-50931 COLOGNE, GERMANY

来源：

EMBO JOURNAL | 1993年 / 12卷 / 11期

关键词：

CD3-ZETA; GENE TARGETING; T-CELL DEVELOPMENT; T-CELL FUNCTION;

D O I：

10.1002/j.1460-2075.1993.tb06120.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CD3zeta is a component of the T cell antigen receptor (TCR) complex and is important for signal transduction. We have established mice selectively lacking CD3zeta but able to express CD3eta, a polypeptide produced from the same locus through alternative splicing, using the method of gene targeting in embryonic stem cells. In homozygous mutant mice, the numbers of thymocytes and peripheral T cells were greatly reduced and the expression levels of TCR on these cells were 5-fold lower than those on wild-type cells. By contrast, TCRgammadelta+ intestinal intraepithelial lymphocytes were not obviously affected by the mutation. T cells from homozygous mutants exhibited an impaired proliferative response. These results imply that CD3zeta has a critical role in the development and signal transduction of T cells in vivo.

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页码：4357 / 4366

页数：10

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