USE OF LOW-TEMPERATURE SCANNING ELECTRON-MICROSCOPY TO OBSERVE FROZEN-HYDRATED SPECIMENS OF NEMATODES

被引:1
作者
WERGIN, WP [1 ]
SAYRE, RM [1 ]
ERBE, EF [1 ]
机构
[1] USDA ARS,BELTSVILLE AGR RES CTR,NEMATOL LAB,BARC W,BELTSVILLE,MD 20705
关键词
COMPLEMENTARY IMAGING; LOW-TEMPERATURE SEM; NEMATODE; PRATYLENCHUS-AGILIS; SEM; STEINERNEMA-CARPOCAPSAE; STEREOLOGY; TECHNIQUE; 3-DIMENSIONAL IMAGING;
D O I
暂无
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Frozen hydrated specimens of Pratylenchus agilis and dauer larvae of Steinernema carpocapsae were observed with low-temperature field emission scanning electron microscopy. This new technique provides information about the surface features of nematodes and also allows specimens to be fractured to reveal their internal structure. Furthermore, both halves of fractured specimens can be retained, examined, and photographed either as two-dimensional micrographs or as three-dimensional images for stereo observation (stereology) or quantitative measurements (stereometry). This technique avoids artifacts normally associated with procedures required to prepare nematodes for examination in the transmission and scanning electron microscopes, such as chemical fixation, dehydration, and sectioning or critical point drying.
引用
收藏
页码:214 / 226
页数:13
相关论文
共 34 条
[1]  
Asahina E, 1959, LOW TEMP SCI B, V17, P51
[2]   CRYOMICROSCOPY OF LIQUID AND SEMILIQUID SPECIMENS - DIRECT IMAGING VERSUS REPLICATION [J].
BACHMANN, L ;
TALMON, Y .
ULTRAMICROSCOPY, 1984, 14 (03) :211-218
[3]  
BIRD A. F., 1991, STRUCTURE NEMATODES, V2nd
[4]  
CURRAN J, 1992, J NEMATOL, V24, P269
[5]  
ECHLIN P, 1970, SCANNING ELECTRON MI, P49
[6]  
Gibbons L. M., 1986, SEM GUIDE MORPHOLOGY
[7]   FREEZE-THAW SURVIVAL OF FREE-LIVING NEMATODE CAENORHABDITIS-BRIGGSAE [J].
HAIGHT, M ;
FRIM, J ;
PASTERNAK, J ;
FREY, H .
CRYOBIOLOGY, 1975, 12 (05) :497-505
[8]  
Huettel R.N., 1985, P155
[9]   FREEZING AND STORAGE OF NEMATODES IN LIQUID NITROGEN [J].
HWANG, SW .
NEMATOLOGICA, 1970, 16 (02) :305-&
[10]  
LAMBERTI F, 1985, CYST NEMATODES